Difficulties with the choice of proper method for determination of serum levels of total testosterone (TT) in European bison males

Authors

  • Elżbieta Czykier Department of Histology and Embryology, Medical University in Białystok

Keywords:

European bison, testosterone, ELISA, RIA

Abstract

The objective of the study was to compare two methods used to determine serum levels of total testosterone (TT) in European bison Bison bonasus (Linnaeus, 1758) males: the radioimmunological method (RIA) and the immunoenzymatic method (ELISA). The study material included sera from 83 European bison males aged 2 months – 19 years, which were culled in the autumn and winter periods (in the post-rutting season), in the years 1993–2002, in the Białowiez˙a Primeval Forest. TT levels were determined using the two methods (RIA and ELISA) in the same animals. The following age groups were distinguished: class I – calves under the age of 1 year, class II –juveniles under the age of 2 years, class III –juveniles under the age of 3, class IV – adult males aged 4–5 years, class V – adult males aged 6–12 years, class VI-old bulls over the age of 12. In our study, serum TT levels measured using the RIA method were lower, higher or similar in comparison with those determined by ELISA in the same animals, although higher concentrations were more frequent when the ELISA method was used. With the RIA method, we obtained 26 results (31.30%) below the bottom limit for this method, i.e. 0.14ng/ml. Included in this number there were 18 animals in class I (56.25%), 6 in class II (37.50%), 1 in class III (14.28%) and 1 in class V (11.11%). The mean serum levels of TT
determined by ELISA were higher than those obtained using RIA in the same age classes, the difference being statistically significant in class I and II (p<0.001), and on the border of statistical significance in class IV and VI (p<0.088 and p<0.047, respectively). 

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Published

2009-09-01

How to Cite

Czykier, E. (2009). Difficulties with the choice of proper method for determination of serum levels of total testosterone (TT) in European bison males. European Bison Conservation Newsletter, 2, 1–10. Retrieved from https://ojs.wisent.org/index.php/czasopismo/article/view/176

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Section

Peer-reviewed articles

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